1/20/2024 0 Comments Sequencing immune repertoire![]() ![]() Learn more about iRepertoire in this video: So instead of working to answer one question, we’re perfecting a way to view the entire immune repertoire. Academic labs are very focused on a single question, whereas he wants the tool that answers all the different questions. I think that happens a little bit faster in industry than academia. When he sees a really good idea, he wants to figure out how to translate it into a product and service to share with somebody else. Miranda: He’s got an entrepreneurial spirit. Jian Han’s motivation for taking this research to industry? Since you joined iRepertoire almost at its founding, what can you tell us about Dr. That’s really incredible because I get to see my methods work in a whole different realm, which is a different kind of joy than doing the research myself. As Director, I get to see people use what I developed in their projects. So having the freedom to do the research and see something no one else has ever seen was the most fun I’ve had. I kind of stepped into that role and was officially promoted later. Then I saw that if we were to continue to grow, somebody needed to step in and begin to manage all these projects and make sure they were completed appropriately. We were doing the research and development and having a lot of fun with it. I was trying to identify antigen specific responses within this immune repertoire sequencing data and developing new methods. Miranda: When I first began, I was purely doing research and development and it was the most fun I ever had. How did you come to be Director at iRepertoire, and what are some of your favorite parts of your position? Han to my dissertation defense, and I had a job offer two days later. And I was like “oh, this is what I want to do.” My advisor must have noticed this sparked something in me and invited Dr. I already knew that I wanted to do something related to human health next, and here was somebody locally who was examining at a systems biology, big picture view these immune receptors and disease. He gave a talk at our lab group about the immune system. Jian Han, MD, Ph.D., about a month before I was to defend my dissertation. Miranda Byrne-Steele: I had the good fortune of meeting Dr. How did you transition from your dissertation work on DNA replication proteins to immunology? from the University of Alabama, Huntsville, where she studied the structure and function of archaeal DNA replication proteins in extremophiles. We spoke with iRepertoire’s Director of R&D and Operations Management Miranda Byrne-Steele, Ph.D., to discuss how her expertise facilitates the development of iRepertoire’s groundbreaking technology. iRepertoire enables researchers to amplify all V(D)J sequences in either B cells or T cells and has the only technology available with the ability to profile all seven adaptive immune chain receptors. They provide a snapshot of the functional status of the immune system. IRepertoire specializes in immune system investigation with its proprietary adaptome sequencing technology. The adaptability of antibody production is driven by V(D)J recombination in antigen receptors that are uniquely expressed on B cells and T cells. Upon infection, antibodies are quickly created to recognize pathogenic antigens for elimination. The adaptive immune system creates a living memory of pathogens you’ve encountered by learning to recognize antigens. The immune system is a complicated network of processes that protect us from diseases. ![]() Immunosequencing in its modern context started being discussed in scientific literature in the early 2010s with the advent of more powerful gene sequencing techniques.A Conversation with Miranda Byrne-Steele, Ph.D. Immunosequencing is a technique utilizing multiplex polymerase chain reaction that allows for the sequencing and quantification of the large diversity of antibody and TCR genes composing an individual's immune repertoire. The makeup and quantity of these proteins is sometimes referred to as an immune repertoire. As an immune system reacts to infections and other events, the number of different antibodies and TCRs it contains changes. The diversity allows for the recognition of a wide array of antigens. ![]() Through V(D)J recombination a number of organisms take a relatively small number of genes coding for antibodies and T-cell receptors (TCRs) and produce a huge diversity of slightly different antibodies and TCRs. In most areas of biology a single gene codes for one or a few possible proteins. Immunosequencing, sometimes referred to as repertoire sequencing or Rep-Seq, is a method for analyzing the genetic makeup of an individual's immune system. ![]()
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